Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method
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چکیده
منابع مشابه
Feulgen reaction study of novel threadlike structures (Bonghan ducts) on the surfaces of mammalian organs.
Threadlike structures on the surfaces of internal organs, which are thought to be part of the Bonghan duct system, were first reported about 40 years ago, but have been largely ignored since then. Recently, they were rediscovered, and in this study we discuss the Feulgen reaction that specifically stains DNA in order to identify these structures on the surface of rabbit livers as part of the Bo...
متن کاملIn vivo visualization of bonghan ducts inside blood vessels of mice by using an Alcian blue staining method.
An in vivo method using Alcian blue (AB) was developed for visualizing floating threadlike tissues inside blood vessels of mice. These novel structures called intravascular Bonghan ducts (IBHDs) are considered as extension of acupuncture meridians. For in vivo imaging of IBHDs, AB solution (pH 7.4) that stains mucopolysaccharides like hyaluronic acid was used. After injecting AB solution into t...
متن کاملUse of Magnetic Nanoparticles to Visualize Threadlike Structures Inside Lymphatic Vessels of Rats
A novel application of fluorescent magnetic nanoparticles was made to visualize a new tissue which had not been detectable by using simple stereomicroscopes. This unfamiliar threadlike structure inside the lymphatic vessels of rats was demonstrated in vivo by injecting nanoparticles into lymph nodes and applying magnetic fields on the collecting lymph vessels so that the nanoparticles were take...
متن کاملContrast-enhancing optical method to observe a Bonghan duct floating inside a lymph vessel of a rabbit.
Novel threadlike structures, so-called Bonghan ducts (BHDs), were recently rediscovered inside large caliber lymphatic vessels using two different staining dyes in rabbits (Janus green B and Alcian blue) and fluorescent nanoparticles in rats. These three methods have the drawback of injecting chemical agents into the lymphatic vessels, which might damage the BHD and hinder further investigation...
متن کاملComposition of the Extracellular Matrix of Lymphatic Novel Threadlike Structures: Is It Keratin?
Background. The lumen of novel threadlike structures (NTSs) is enclosed by a single layer of endothelial cells surrounded by extracellular matrix (ECM). We hypothesized that collagen may be a component of the ECM associated with lymphatic NTSs. Methods. Six female New Zealand white rabbits were anesthetized, and the NTS structures within lymphatic vessels were identified by contrast-enhanced st...
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ژورنال
عنوان ژورنال: The Anatomical Record Part B: The New Anatomist
سال: 2005
ISSN: 1552-4906,1552-4914
DOI: 10.1002/ar.b.20076